Ocular Safety Test – For Eye and Facial Products

Ocular Safety Test – For Eye and Facial Products

The objective of the Ocular Safety test is to determine the potential of a facial or eye area product to cause ocular irritation. This test is recommended for all cosmetic products and face creams that could potentially migrate into the eyes during application or normal wear. This study may be combined with a Dermal Irritancy Test for products such as foundations, moisturizers, sunscreens, and products such as shampoos or conditioners.

Ocular Safety Test – For Eye and Facial ProductsThis Ocular Safety test is also known as the Neutral Red Release (NRR) and is a lab based cell test. No animals or humans are involved Neutral Red Release (NRR)

Title Assessment of the irritant potential of a test element by direct application to fibroblasts of rabbit cornea by the neutral red release method
Reference Adapted from Reader (Tox. In Vitro, 1990, 4, 264-266)
Published in the Official Journal of the French Republic of December 30, 1999
Objective To assess quantitatively the cytotoxicity of a test element
Products containing surfactants are diluted to 5% before the test
Test system Fibroblasts of rabbit cornea – SIRC
Schedule Duration of the study: 3 days
Beginning: 1 week upon receipt of the sample
Report: 2-3 weeks after the end of the study / 1 week after the end of study
Quantity 2 x 20 g
Methodology Determination of the cytotoxicity after contact of the test element (5 dilutions) with a monolayer of cells marked by a vital dye (Neutral Red) by calculation of the percentage of cell death and the IC50 or concentration of test element that inhibited of 50 % the survival and cell growth.
Procedure D-1 or D-2: Cells seeding (24h/48h)Preparation of the dye and the revealing solution
D1: Cell staining
Contact with the test element dilutions
Washing
Revelation of the cytotoxicity
Reading